Environmental and human-health risks of As in soils with abnormal arsenic levels located in irrigated agricultural areas of Paracatu (MG), Brazil.

The municipality of Paracatu (Brazil) is notorious for its large irrigated agricultural area and by abnormal arsenic (As) levels in selected soils of the region. Concerns regarding As exposure via ingestion of water and food are frequent, yet little is known about the behavior of arsenic in irrigated agricultural soils, as well as on As bioaccessibility/bioavailability in agroecosystems of this region. This work evaluated total and available As in agricultural soils cultivated under irrigation and in soils under native vegetation in Paracatu. We also assessed reactive arsenic fractions and As bioaccessibility in the soil, as well as arsenic levels in plant shoots to estimate As risks in these agroecosystems. Soil (different depths) and plant tissue samples were collected in 6 irrigated agricultural areas (CA1 to CA6) and 4 reference areas (RA1 to RA4). Total soil-As did not differ between soil depths, reinforcing that the source of As in agricultural soils is natural. This was evident when counterpointing arsenic and phosphorus contents at different soil depths, as both accumulate on the surface of oxidic soils when added to agroecosystems by anthropogenic routes (e.g., phosphate fertilization for P and irrigation for As). Available As levels in soils and plants were very small (below detection limit). Furthermore, all soils presented very low oral As bioaccessibility. Our findings revealed that the irrigated soils are not As polluted due to the low enrichment and accumulation of arsenic, as well as the prevalence of low ecological risks. There is no non-carcinogenic risk for the local population, except for children in RA2. The estimated carcinogenic risk for children followed the order RA2 > CA3 > CA4 > RA3 > CA2, and for adults, RA2 > CA3. Ultimately, the strategy of comparing the behavior of P and As in the soils of this study proved to be efficient in showing that there are no major risks to humans and the environment in the investigated area. However, periodic monitoring of As bioavailability in these areas is recommended.

Investigating arsenic toxicity in tropical soils: A cell cycle and DNA fragmentation approach.

Arsenic (As) is a metalloid and a toxicant that is found naturally in many environmental compartments, soils included. Soils with high levels of As occur worldwide and might pose a threat not only to humans, but also to many ecosystems. Considering the scarcity of studies regarding cytogenotoxic effects of model plants in As-contaminated soil, mainly in tropical areas, this study proposes the use of Allium cepa root tip bioassays for a fast-track assessment of As toxicity in tropical soils. For this end, root tip cells of A. cepa were exposed to an Oxisol, an Inceptisol and a Tropical Artificial Soil (TAS) contaminated with increasing doses of As (0, 8, 14.5, 26, 46.5, 84, 150, and 270 mg kg-1). The effects of As on cell cycle, micronucleus formation, and DNA fragmentation were evaluated. In general, root tip cells exposure to As increases the frequency of chromosome abnormalities and micronucleus, in turn, decreasing the frequency of mitotic index. As-treated cells also presented an increase in the percentage of DNA damage observed in comet assay. Overall, the effects of As in TAS were more pronounced, than in the Oxisol, being the Inceptisol the less toxic. A discussion of each As effect in cells and the link with the soil type is presented and reveals that clastogenic effects of As in A. cepa cells seemed to be the mode of action of this soil contaminant.

Nucleolar alterations are reliable parameters to determine the cytogenotoxicity of environmental pollutants.

Pollution generated by deposition of industrial activity waste in the environment without due care can lead to serious environmental consequences. Bioassays in higher plants are means of understanding the cytogenotoxic effects of these substances. In the present work, Allium cepa L. was used as a model species to assess nucleolar changes induced by environmental pollutants. The substances used were Methyl Methane Sulfonate (MMS), cadmium (Cd), Spent Potliner (SPL) and the herbicide Atrazine. Water was used as a negative control. The silver-stained nucleolar organizer region (AgNOR) assay was used making it possible to evaluate how nucleolar parameters (number of nucleoli per nucleus and nucleoli area) behave when facing stress caused by such pollutants. The results obtained showed a variation in the observed parameters: an increase in the number of nucleoli in the treated cells and tendency to a reduction in nucleolar area, indicating that the tested pollutants may have impaired nucleolar activity. In addition, it was possible to establish a relationship between the behavior of the nucleolus with other changes as plantlet growth, cell proliferation, and DNA damage.

Amending potential of organic and industrial by-products applied to heavy metal-rich mining soils.

Mining activities promote the development of economies and societies, yet they cause environmental impacts that must be minimized so that their benefits overcome the likely risks. This study evaluated eco-friendly technologies based on the use of low-carbon footprint wastes and industrial by-products as soil amendments for the revegetation of Zn-mining areas. Our goal was to select adequate soil amendments that can be used to recover these areas, with a focus on low-cost materials. The amendments - limestone, sewage sludge, biochar, and composted food remains - were first characterized concerning their chemical composition and structural morphologies. Soil samples (Entisol, Oxisol, Technosol) from three different areas located inside an open-pit mine were later incubated for 60 days with increasing doses of each soil amendment, followed by cultivation with Andropogon gayanus, a native species. The amendments were able to change not only soil pH, but also the phytoavailable levels of Cd, Zn, and Pb. Limestone and biochar were the amendments that caused the highest pH values, reducing the phytoavailability of the metals. All amendments improved seed germination; however, the composted food remains presented low levels of germination, which could make the amendments unfeasible for revegetation efforts. Our findings showed that biochar, which is a by-product of the mining company, is the most suitable amendment to enhance revegetation efforts in the Zn-mining areas, not only because of its efficiency and cost, but also due to its low carbon footprint, which is currently the trend for any "green remediation" proposal.